A simple, fast, precise, accurate and specific HPTLC method was developed and validated for the simultaneous estimation of Montelukast sodium (MTKT) and Desloratadine (DLOR) from their combined tablet dosage form. Chromatographic separation was achieved on aluminum plates precoated with silica gel G60F254, the solvent system consisted of Methanol: chloroform: ammonia 2.5:7.5:0.1 (v/v/v) as mobile phase. Detection was performed densitometrically at 282 nm. The RF of Montelukast sodium and Desloratadine were 0.64 ± 0.01 and 0.18 ± 0.01 respectively. Linearity was observed in the concentration range of 800-2400 ng/spot for Montelukast sodium and 400-1200 ng/spot for Desloratadine. Percent recoveries obtained for both the drugs were 99.48 ± 0.66% – 101.20 ± 0.35% and 99.15 ± 0.70 – 101.16 ± 0.38%, respectively. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. The developed method can be used for the routine analysis of Montelukast sodium and Desloratadine from their combined tablet dosage form.
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